Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system
نویسندگان
چکیده
منابع مشابه
Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.
The phiC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition sites. Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in embryos. Intramolecular recombination in S2 cells on transf...
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چکیده ندارد.
Fluorescent reporters for the ubiquitin-proteasome system.
Regulated turnover of proteins in the cytosol and nucleus of eukaryotic cells is primarily performed by the ubiquitin-proteasome system (UPS). The UPS is involved in many essential cellular processes. Alterations in this proteolytic system are associated with a variety of human pathologies, such as neurodegenerative diseases, cancer, immunological disorders and inflammation. The precise role of...
متن کاملIdentification of a Specific Pseudo attP Site for Phage PhiC31 Integrase in Bovine Genome
Background: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous DNA into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene. Objectives: Identification of a novel pseudo attP site. Materials and Methods...
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ژورنال
عنوان ژورنال: genesis
سال: 2010
ISSN: 1526-954X,1526-968X
DOI: 10.1002/dvg.20637